Practical Methods for Biocatalysis and Biotransformations [Englisch] [Gebundene Ausgabe]

"Chapters cover key biotransformations through the literature, detailing the procedures used, the sources of the starting materials and reagents, references, and tips and advice, where appropriate." (JACS, 2010)
 
"It starts which has a review in the current use of biocatalysis within the pharmaceutical industry. Although this is centered on a large pharmaceutical company (GlaxoSmithKline), it covers a broad selection of aspects of the industry and offers insightful comparisons of biocatalysis with 'traditional' organic synthesis." (Chemistry World, July 2010)
 
"I can suggest to any any those who are interesting in carrying out biotransformations inside laboratory, and it should help persuade people that haven't used a biotransformation within an organic synthesis, to use one to the first time." (Organic Process Research & Development Journal, 2010)

Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a massive range of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities.
 
Practical Methods for Biocatalysis and Biotransformations is really a "how-to" guide focussing on commercially available enzymes and strains of microorganisms which are readily from culture collections. The way to obtain starting materials and reagents, hints, tips and safety advice (where appropriate) are presented to ensure, so far as possible, that this procedures are reproducible. Comparisons to alternative methodology get and relevant references for the primary literature are cited. Contents include:
* Introduction for the use of biocatalysts in the pharma industry
* introduction to molecular biological techniques for biocatalyst development
* Lipase and protease methods including enzyme supports and dynamic kinetic resolutions
* Nitrile hydrolases and hydratases
* Alcohol dehydrogenases and co-factor recycling
* Epoxide hydrolases
* Amino acid synthesis
* Whole cell processes
* Other biocatalysts, including monoamineoxidases, aminotransterases, and lyases
 
Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.



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